SARS-CoV-2 (COVID-19) 3CL-Mpro Protein, unmodified

€430.00 €490.00

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SKU: P2020-027 trenzyme

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Description

Cysteine protease 3CL-Mpro of SARS-CoV-2 (COVID-19) that cleaves the transcribed viral polyprotein into several functional proteins at two self-cleavage sites.

Overview

  • Product Name: SARS-CoV-2 (COVID-19) 3CL-Mpro Protein, unmodified
  • Catalog No.: P2020-027
  • RefSeq Links: NC_045512.2; MN908947.3; YP_009724390.1; QHD43416.1; GeneID: 43740568; UniProt: P0DTD1
  • Synonyms: 3CL Mpro; 3CL Pro; 3CL protease; 3C-like main protease; SARS-CoV-2; coronavirus; 2019-nCoV; COVID-2019; COVID-19
Cellebrity Kolben Cell Cartoon trenzyme

Customer Testimonial

“In our COVID-19 projects, we have had very good experience with the SARS-CoV-2 proteins produced by trenzyme: rapid and reliable production of the functional proteins from different cell lines continued to provide first-class support for our projects.”


Dr. Peter Rauch
CANDOR Bioscience GmbH, Wangen, Germany

Sequence Information

  • Species: SARS-CoV-2; Wuhan seafood market pneumonia virus
  • Tags: Tag-free
  • Sequence without tags (AA 1-306):
    SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIR
    KSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNG
    SPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGN
    FYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYE
    PLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQC
    SGVTFQ

Product Information

  • Expression Host: E. coli
  • Formulation: PBS, pH 7,4; contains Glycerol as protectant
  • Format: Liquid, stored and shipped at -80°C
  • Purity: > 90% as determined by SDS-PAGE

Background Information

The new coronavirus SARS-CoV-2 expresses two proteases, the papain-like protease (PLpro) and 3C-like protease (3CLpro). Both belong to the group of cysteine proteases, as they have a cysteine residue at their catalytic site. Their main function is the processing of the viral polyprotein, that contains two cleavage sites to build up the viral replicase complex. Additionally, PLpro has the ability of removing ISG15 and ubiquitin from viral proteins expressed in the cell, this enables evasion from the innate immune response by the host. This presents an interesting target for drug development, as it would not only inhibit the viral replication but would also prevent the massive immunological response resulting of the over-activation of the host´s immune system, that can lead to damaging of uninfected cells and therefore worsening of the patient´s condition.
Our protein contains no additional amino acids at the N-terminus like proteins from competitors. Therefore, the protease has the authentic N-terminus which is part of the active site of the protein. N-terminal His-Tag was removed by a proteolytic digest producing an authentic N-terminus to ensure highest proteolytic activity.


SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

SARS-CoV-2 (COVID-19) 3CL-Mpro Protein, unmodified SDS Page
SARS-CoV-2 (COVID-19) 3CL-Mpro Protein, unmodified Histogram

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