Cleavage and tag control protein

€310.00

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SKU: P2020-131 trenzyme

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Description

The cleavage and tag control protein is a versatile protein consisting of various cleavage sites for proteases including TEV, Enterokinase, Thrombin, Factor Xa and PreCission. Protease cleavage results in generation of two fragments that have similar molecular weights, which are as follows: - TEV cleavage: 42.8 kDa and 47.7 kDa - Enterokinase: 43.5 kDa, 20.1 kDa and 26.9 kDa - Thrombin: 43.9 kDa and 46.6 kDa - Factor Xa: 44.3 kDa and 46.2 kDa - PreCission: 45.3 kDa and 45.3 kDa Moreover, the 90.5 kDa fusion protein is equipped with multiple tags such as Twin-Strep-, HA-, FLAG-, and His-tag, for instance, that may serve as positive control performing specific Western blot analyses or any functional assay. Since the protein is fused to GFP, it is additionally applicable as fluorescence control. Thus, the cleavage and tag control protein provides an excellent tool to monitor protease cleavage reactions and specific Western blot analyses as well as fluorescence experiments.

Overview

  • Product Name: Cleavage and tag control protein
  • Catalog No.: P2020-131
  • Synonyms: control protein, reference protein, cleavage control protein, multiple tag control protein, universal reference protein

Sequence Information

  • Species: artificial
  • Tags: Twin-Strep, HA, T7, Avi, FLAG, and His-tag; N-terminal MBP and C-terminal sfGFP fusion

Product Information

  • Expression Host: E. coli
  • Formulation: PBS; pH 7.4
  • Format: Liquid, stored and shipped at -80°C
  • Purity: > 99 % (measured by densitometry of Coomassie stained gel, see below)

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

SDS-PAGE of Test Cleavage Histogram (of marked lane in gel picture) of Test Cleavage

Test Cleanage

Description of Test Cleavage

SDS-PAGE of Test Cleavage Description of Test Cleavage

Test Cleavage

Description for Test Cleavage

SDS-PAGE of Test Cleavage Description of Test Cleavage

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