Proteinase K, powder formulation

• Species: Tritirachium album
• Sequence: DNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 1 µg λ DNA for 6 hours at 37°C. RNase is not detected in quality control procedure of incubation 40 µg Proteinase K with 2 µg RNA for 2 hours at 37°C. This preparation is considered as RNase and DNase free.

• Expression Host: Pichia pastoris
• Formulation: Supplied as a lyophilized powder
• Format: Below 4 °C storage temperature recommended. Short term shipping and storage at ambient temperatures do not cause activity loss.
• Purity: > 95% as determined by SDS-PAGE

The recombinant enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant preparation has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
Crystal structure data indicate that the enzyme belongs to the subtilisin family, which is characterized by a catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴) in the active site.
Proteinase K does not exhibit pronounced cleavage specificity, and the preferred cleavage site is the peptide bond at hydrophobic amino acids.

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