Recombinant protein of the receptor binding domain (RBD) Mutant (K417N, L452R, T478K) of SARS-CoV-2 (COVID-2019) Spike S1 from Wuhan pneumonia virus with C-terminal Fc/His-Tag. The mutations K417N, L452R and T478K are characteristic for the SARS-CoV-2 virus variant AY.1/AY.2/AY.3 which were initially reported in America with a single reclassified case in the UK and India in August 2021. AY.1/AY.2/AY.3 are also known as Delta Plus variant, sub-lineages of Delta. The mutations are affecting the receptor binding domain (RBD) of the spike protein, which the virus uses to bind to human cells receptors and enter them. Due to the mutations, the virus is allowed to bind with higher affinity to human ACE2 receptor which results in increased transmissibility of the SARS-CoV-2 virus.
“In our COVID-19 projects, we have had very good experience with the SARS-CoV-2 proteins produced by trenzyme: rapid and reliable production of the functional proteins from different cell lines continued to provide first-class support for our projects.”
Dr. Peter Rauch CANDOR Bioscience GmbH, Wangen, Germany
The spike (S) glycoprotein of coronaviruses is essential for binding of the virus to the host cell at the beginning of the infection process. The target protein is also a major immunogen and a possible target for entry inhibitors. The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (RBD) responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2). Several mutants of the spike protein are known, including a novel SARS-CoV-2 lineage AY.1/AY.2/AY.3. Compared to the previously circulating variants, the mutations L452R and T478K of the SARS-CoV-2 Spike S1 (RBD) may cause a stronger affinity of the spike protein to hACE2 and also conferring an increasing ability to evade the hosts’ immune system. Furthermore, the Delta Plus variant has an additional K417N amino acid mutation. This mutation seems to have no significant impact on the clinical spectrum of disease and not more transmissible than Delta.