SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), liquid formulation

€300.00

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SKU: P2020-001 trenzyme

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Description

Recombinant protein of the receptor binding domain (RBD) of SARS-CoV-2 (COVID-19) Spike S1 from Wuhan pneumonia virus with C-terminal His-Tag, liquid formulation.

Overview

  • Product Name: SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), liquid formulation
  • Catalog No.: P2020-001
  • RefSeq Links: NC_045512.2; MN908947.3; YP_009724390.1; QHD43416.1; GeneID: 43740568; UniProt: P0DTC2
  • Synonyms: SARS-CoV-2; coronavirus; SARS-CoV-2 spike RBD; SARS-CoV-2 spike protein; 2019-nCoV; COVID-2019; COVID-19

Customer Testimonial

“In our COVID-19 projects, we have had very good experience with the SARS-CoV-2 proteins produced by trenzyme: rapid and reliable production of the functional proteins from different cell lines continued to provide first-class support for our projects.”


Dr. Peter Rauch
CANDOR Bioscience GmbH, Wangen, Germany

Sequence Information

  • Species: SARS-CoV-2; Wuhan seafood market pneumonia virus
  • Tags: His-Tag, C-terminal
  • Sequence without tags (AA 319-541):
    MRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTF
    KCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWN
    SNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGF
    QPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Product Information

  • Expression Host: human, HEK293
  • Formulation: PBS, pH 7,4
  • Format: Liquid, stored and shipped at -80°C
  • Purity: > 85% as determined by SDS-PAGE

Background Information

The spike (S) glycoprotein of coronaviruses is essential for binding of the virus to the host cell at the beginning of the infection process. The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein is responsible for membrane fusion and is therefore required for virus entry and cell fusion. The target protein is also a major immunogen and a possible target for entry inhibitors.
The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (RBD) responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2). The S2 subunit mediates fusion between the viral and host cell membranes. The S1 RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.


SARS-CoV-2 Spike S1 (RBD) recognizes hACE2 (ECD) with an affinity constant of 1 nM as verified by biolayer interferometry

SARS-CoV-2 Spike S1 (RBD) is highly pure and forms stable monomers of 28 kDa as verified by SEC

Biolayer interferometry binding analysis graph Determination of elution profile by absorbance at 289 nm (blue line).

Biolayer interferometry binding analysis (green lines) of hACE2 (ECD, processed), Tag-free to immobilized SARS-CoV-2 Spike S1 (RBD), His-Tag on Ni-NTA Dip and Read™ Biosensors. Grey lines correspond to a global fit of the data using a 1:1 binding model.
Device: Octet RED96e, ForteBio.

Size exclusion chromatography (SEC) of purified SARS-CoV-2 Spike S1 (RBD), His-Tag protein, determination of elution profile by absorbance at 280 nm (blue line).

 

Fluorescence chromatogram

Activity of SARS-CoV-2 S1(RBD)

Analysis of released N-glycans by HILIC (example data) of SARS-CoV-2 Spike S1 (RBD) Graphic showing activity of SARS-CoV-2 S1(RBD)

Analysis of released N-glycans by HILIC (example data). More and lot specific analytical data available on request (provided by our partner  Biofidus AG).

Activity of different SARS-CoV-2 S1(RBD) lots was determined using a Sandwich-ELISA with antibodies from Sino Biological Europe GmbH (coat: #40150-D003 and detection: #40150-D001-H).

Stability during storage at 4°C

Stability during storage at 4°C determined by functional ELISA.

Stability during storage at 4°C determined by functional ELISA (binding to hACE2 (ECD) – Cat# P2020-016; detection by mAb CR3022).

 

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

SDS-PAGE of SARS-CoV-2 Spike-S1 (RBD) Protein Histogram (of marked lane in gel picture) of SARS-CoV-2 Spike S1 Protein

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