human B7-2/CD86
€270.00
Price excl. shipping costs excl. VAT. For more information, see our shipping policy
SKU: P2020-153 trenzyme
Description
B7-2, also known as cluster of differentiation 86 (CD86), is a cell surface glycoprotein expressed on antigen-presenting cells (APCs) and plays a crucial role in regulating adaptive immune responses. As co-stimulatory molecule, B7-2 binds to CD28 expressed on the surface of T cells and thereby, provides a second signal that is inevitable for T cell activation, proliferation and differentiation into effector and memory T cells. Inhibitory signals induced by interaction of B7-2 with the cytotoxic T lymphocyte antigen-4 (CTLA-4) limit initial T cell activation in secondary lymphoid organs in order to prevent excessive immune stimulation. Modulating the B7-2/CD28 and B7-2/CTLA-4 interactions can have significant effects on immune responses and thus, represents a promising strategy to treat various autoimmune diseases and cancer.
Overview
-
Product Name: human B7-2/CD86
-
Catalog No.: P2020-151: GFP/His-Tag, P2020-152: Fc/His-Tag, P2020-153: His-Tag
-
RefSeq Links: UniProt: P42081; HGNC:1705; NX_P42081; NP_787058.4; NM_175862.4; PDBe 8hxb
-
Synonyms: CD86, CD86 antigen, T-lymphocyte activation antigen CD86, B7-2, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD28LG2, LAB72, MGC34413
Customer Testimonial
“We highly valued the fast and excellent communication and the high flexibility of the team! For any future project, we will preferably entrust in trenzyme’s protein production services.”
Dr. Thore Hettmann
Probiodrug AG, Halle/Saale, Germany
Sequence Information
- Species: Homo sapiens
-
Tags:
P2020-151: GFP/His-Tag
P2020-152: Fc/His-Tag
P2020-153: His-Tag -
Sequence without tags (AA 26-247):
MLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMG
RTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPI
SNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLS
VSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP
Product Information
-
Expression Host: HEK293
-
Formulation: PBS; pH 7.4
- Format: Liquid, stored and shipped at -80° C
-
Purity: > 95 % as determined by SDS-PAGE
- Application: ELISA, WB, Immunostaining, Functional assay
Background Information
The cell surface glycoprotein B7-2, also known as cluster of differentiation 86 (CD86), belongs to the B7 family of co-stimulatory molecules, which are expressed on antigen-presenting cells (APCs), including dendritic cells, macrophages and B cells. Resting APCs express low levels of B7 molecules, but various stimuli, such as microbial products and cytokines produced during innate immune reactions against pathogens, induce an increased expression of B7 molecules in order to promote adaptive immune responses. This regulated expression ensures that T cells are activated only when needed. When T cells encounter antigens displayed by APCs, which is the first signal required for complete T cell activation, B7-2 interacts with CD28 providing a co-stimulatory signal, the second crucial signal. T cell activation leads to cytokine production and proliferation of T cells, as well as differentiation into effector and memory T cells. This interaction is counterbalanced by the cytotoxic T lymphocyte antigen-4 (CTLA-4), which is expressed on T cells after antigen recognition and has a higher affinity for B7-2 than CD28. CTLA-4 provides inhibitory signals leading to downregulation of immune responses. Dysregulation of B7-2 causes autoimmune diseases, inflammatory disorders and cancer. Therefore, therapeutic strategies targeting B7-2 or its interactions are being explored to dampen autoimmune diseases and to enhance anti-cancer immunity.
Additional information B7-2/CD86 GFP/His-Tag
|
SDS-PAGE/Coll. Coomassie |
Histogram of marked lane in gel picture |
 |
 |
Additional information B7-2/CD86 Fc/His-Tag
|
SDS-PAGE/Coll. Coomassie |
Histogram of marked lane in gel picture |
 |
 |
Additional information B7-2/CD86 His-Tag
|
SDS-PAGE/Coll. Coomassie |
Histogram of marked lane in gel picture |
 |
 |
